Study of bio-samples with Small Angle X- Ray Scattering and Electron Microscopy
The aim of the project is the study of bio – materials by the methods of Small/Wide X – Ray Scattering (SAXS/WAXS) and electron microcopy. With these techniques studied healthy and cancerous tissues, bone nanostructure, cerebrospinal fluid, drug delivery in bones and mechanical stresses in bones (compression, torsion, 4 point bending).
To date, have been analyzed breast cancer tissues and a critical amount of rabbit bones. Particularly impressive are the results of measurements taken from rabbit bones with SAXS technique. Already made some preliminary SAXS experimental on rabbit bones, some of which were treated with the drug Protelos. The study has found that in the treated bones, the substance collects at the ends of the bone instead of in the center.
The main novelty of the project is the innovative use of in-situ characterization techniques, which to the best of our knowledge has never been done before. In situ techniques are accepted to be research orientated and very rare in routine analysis. This is because the instruments needed for in–situ studies are usually high–cost and high–tech equipment’s that are overly burdened with routine experiments. Yet, in-situ studies provide information not only on the structure of the materials under investigation but also on the mechanism of the phenomena and processes that take place inside the structure. This latter knowledge is of critical importance in the context of modern medical challenges in drug delivery, biomechanics, etc.
The study approximates two issues high demand and scientific value that is: a) the development of a diagnostic method of CA and b) biomechanics of bones. The project carried out, under the supervision of Prof. E. Sivrides, Scholl of Medicine (D.U. Th. ) and the experiments performed in the CISS lab of KavTech.
Lewis, R.A., Rogers, K.D., Hall, C.J., Towns-Andrews, E., Slawson, S., Evans, A., Pinder, S.E., Dance, D.R., “Breast cancer diagnosis using scattered X-rays”, Journal of Synchrotron Radiation 7 (5),(2000), pp. 348-352.
Liu, Y., Manjubala, I., Schell, H., Epari, D.R., Roschger, P., Duda, G.N., Fratzl, P., “Size and habit of mineral particles in bone and mineralized callus during bone healing in sheep”, (2010) Journal of Bone and Mineral Research, 25 (9), pp. 2029-2038.
Ballarre, J., Manjubala, I., Schreiner, W.H., Orellano, J.C., Fratzl, P., Ceré, S., “Improving the osteointegration and bone-implant interface by incorporation of bioactive particles in sol-gel coatings of stainless steel implants”,(2010) Acta Biomaterialia, 6 (4), pp. 1601-1609.
Kavala Institute of Technology
St. Lucas, 65404, Kavala, Greece
Tel: +30 2510 462247